The kapa sybr fast qpcr kit is supplied as a 2x master mix with integrated antibody mediated hot start sybr green i fluorescent dye mgcl 2 dntps and stabilizers. For the rotor gene q and other rotor gene cyclers we recommend using the rotor gene sybr green pcr kit which has been specially developed for fast cycling on these. Reproducible consistent amplification across a wide dynamic range fast sybr green master mix contains all of the components excluding the template and primers in a convenient 2x master mix amplitaq fast dna polymerase up a highly purified dna polymerase designed to allow instant hot start minimizing non specific product formation.
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