Storage this product is specifically designed for the following instruments. 2x bimaketm sybr green master mix component templatec forward primerd 5 µm reverse primerd 5 µm rox reference dye distilled water dh 2o total reaction volumec amount per reaction µl amount per reaction µl variable 2 5 2 5 1 add to 50 50 variable 1 1 0 4 add to 20 20 1 100 ng 0 2 1 µm 0 2 1 µm 1x protocol component 5 ml 200. Fast sybr green master mix protocol purpose of the kit about the kit fast sybr green master mix supplied in a 2 concentration is a convenient premix to perform real time pcr using sybr green i dye.
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